bet365 paid out £45m as part of the Sub On, Play On offer throughout the World Cup group stage.
The offer, which sees player bets roll on to their replacement if the player is subbed off, had led to an extra £30m in payouts across the first 11 days of the World Cup, and now approaches £50m at the knockout stage.
England's first game saw plenty of Anthony Gordon backers have their bets settled as winners as replacement Marcus Rashford found the net, though Bradley Barcola replacing Ousmane Dembele in France's opener against Senegal remains the biggest payout of the tourmament.
bet365’s Steve Freeth said: "The costliest ones were earlier in the tournament, but Soufiane Rahimi replacing Ismael Saibari after he had scored for Morocco v Haiti stung a lot.
"The substitute scoring and producing an assist had the Bayern Munich-bound forward Saibari scoring two goals and an assist with our offer, despite only having one goal in the record books. The Moroccan No.11 has been extremely popular due to his flying start at the tournament.
"It gets even better for bet365 customers during the knockout stages as extra-time is included in all player props."
France v Senegal | Bradley Barcola replacing Ousmane Dembele |
Netherlands v Sweden | Crysencio Summerville replacing Donyell Malen |
New Zealand v Egypt | Mahmoud Trezeguet replacing Omar Marmoush |
England v Croatia | Marcus Rashford replacing Anthony Gordon |
Morocco v Haiti | Soufiane Rahimi replacing Ismael Saibari |