Ecuador and Curacao will both be aiming to get on the board in Group E when they battle it out in their second fixture.
While Ecuador conceded a 90th minute winner against Ivory Coast in their World Cup opener, Curacao made history by becoming the smallest ever nation to appear on the global stage and Livano Comenencia scored a memorable goal in their 7-1 defeat to four-time winners Germany.
Ecuador v Curacao will be available to watch live in the UK on BBC One.
Eligible customers in the UK, Ireland, Greece, Netherlands and Australia will also be able to bet on and watch the World Cup at bet365.
Ecuador v Curacao will be played at Arrowhead Stadium in Kansas City and kick-off is at 01:00 BST on Sunday 21st June.
This is the first competitive meeting between Ecuador and Curacao.