A place in the last 16 against Switzerland is up for grabs as Colombia and Ghana lock horns in Kansas City.
Colombia were impressive as they took seven points from a possible nine to finish above Portugal in their World Cup group, while Ghana secured their place in the knockout stages with a late victory over Panama and a goalless draw with England.
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Colombia v Ghana will be available to watch live in the UK on ITV1 and ITVX.
Eligible customers in the UK, Ireland, Greece, Netherlands and Australia will also be able to bet on and watch the World Cup at bet365.
Colombia v Ghana will be played in Kansas City and kick-off is at 02:30 BST on Saturday 4th July.
This is the first competitive meeting between Colombia and Ghana.
Read the Colombia v Ghana tips and predictions here.