Both sides will be aiming to build on impressive opening performances when Colombia and DR Congo do battle in the early hours of Wednesday morning.
Colombia took a huge step towards qualifying for the knockout stages with a comfortable 3-1 victory over Uzbekistan in their World Cup opener, while DR Congo made history as they held Portugal to a 1-1 draw in Houston.
Colombia v DR Congo will be available to watch live in the UK on ITV 1 and ITVX.
Eligible customers in the UK, Ireland, Greece, Netherlands and Australia will also be able to bet on and watch the World Cup at bet365.
Colombia v DR Congo will be played at Estadio Guadalajara and kick-off is at 03:00 BST on Wednesday 24th June.
This is the first competitive meeting between Colombia and DR Congo.