Fictional grassroots team Royal Oak FC were beaten 2-1 by bitter rivals Nag's Head in the quarter-finals of the Sheffield Imperial Cup on Sunday afternoon.
The game was billed as the 'biggest game in Sunday league football' and took place in front of over 2,000 supporters at the home of Sheffield FC, the world's oldest football club.
The build up to the match had been dominated by the mid-season transfer of 'Wynny' from the Royal Oak to the Nag's Head, with the forward relishing in the role of pantomime villain.
The Nag's Head took the lead on the hour through a brilliant left-footed free-kick, before doubling their advantage in the 72nd minute as the Royal Oak failed to clear a corner.
Paul Sampson, Steve Bracknall and Bob the Bucket sensed a comeback five minutes later as captain 'Tommy' scored from the penalty spot.
The Royal Oak were then awarded a second penalty six minutes from time following a controversial handball, but 'Tommy' saw his effort saved, before his retake was also saved by the Nag's Head 'keeper.
Bracknall watched on as the Royal Oak players were unable to force an equaliser, with their rivals booking their place in the Sheffield Imperial Cup semi-finals.
The match was broadcast live on BBC Radio Sheffield and also attracted a peak audience of almost 50,000 via the 'Game's Gone' YouTube channel, home of Steve Bracknall's podcast.
Some of the supporters in attendance at Sheffield FC are believed to have travelled from as far as the United States to be present at the historic sunday league fixture.
In addition to both teams being fictional, Bracknall and Sampson are also characters that have been devised by the Sheffield duo.
Sampson is better known as Jon McClure of Sheffield band Reverend and the Makers, while Bracknall's character is played by Jon's brother Chris.
The latter also achieved fame by appearing on the front cover of Arctic Monkeys' debut album Whatever People Say I Am, That's What I'm Not two decades ago.