Gillingham and Crystal Palace U21 will both go looking for their opening EFL Trophy points of the season in their Southern Section Group D clash.
The Gills were beaten by Peterborough in last month's opening game, while Palace's U21s were edged out by Stevenage.
Both teams will know their hopes of progress in the competition rest heavily on a positive outcome in this game.
The game between Gillingham and Crystal Palace U21 will be broadcast on Sky Sports+ in the UK.
The EFL Trophy clash between Gillingham and Crystal Palace U21 kicks off at 19:00 on Tuesday 24th September and will be played at Priestfield Stadium in Gillingham.
Gillingham find themselves joint top of League Two following successive wins over Tranmere Rovers and Notts County - Elliott Nevitt netted the only goal in Saturday's win at Meadow Lane.
Crystal Palace U21 are unbeaten in four since that EFL Trophy loss to Stevenage, though the three most recent of those games - against Leeds U21, Real Sociedad B and Reading U21 - have all ended level.
This will be the first meeting of these two sides.
Conor Masterson (Achilles), Tim Dieng (knee), Aaron Rowe (hamstring), Jake Turner (ankle) and Josh Andrews (quad) were all ruled out of the weekend win at Notts County and remain doubts.
Bradley Dack has been out with a hamstring issue, but could make the bench, while Ethan Coleman is back in contention after serving a suspension.
Jake Grante, Joe Sheridan and goalscorer Jemiah Umolu were all restored to the Palace U21 line-up for the 1-1 draw with Reading U21 on Friday, as Caleb Kporha, Sean Grehan and David Obou dropped out.
Boss Darren Powell may opt for further changes, though no injury problems were reported after that clash with the Royals.