After drawing their opening EFL Trophy game 3-3 with Cambridge United, Bromley host Chelsea U21 in their second group clash.
Bromley picked up an extra point by beating Cambridge on penalties and now they will aim for a home victory to boost their chances of progressing past the group stage.
This is the Chelsea youngsters' first Southern section Group C game as the Blues' academy players look to impress against the League Two side.
The game between Bromley and Chelsea U21 will be available to watch on Sky Sports+ in the UK.
The EFL Trophy game between Bromley and Chelsea U21 takes place at Bromley's Hayes Lane home in London and kicks off at 19:45 on Tuesday 24th September.
Bromley are 18th in League Two with just two wins from their first seven league games and they come into the EFL Trophy clash against Chelsea on the back of consecutive defeats to Notts County (4-2) and Grimsby Town (1-0).
Chelsea U21s are 15th in Premier League 2 following a 2-1 defeat over the weekend against West Brom. The youngsters have won just one of their four league games this season.
Tuesday's clash will be the first meeting between Bromley and Chelsea U21s.
Forward Marcus Dinanga is set to come into the Bromley side after he scored twice in the first group game as boss Andy Woodman prepares to make several changes to give some of his fringe players a chance.
Omar Sowunmi and Lewis Leigh both came off the bench in the weekend League Two defeat at Grimsby and will hope to start.
Reiss Russell-Denny was on target against the Baggies on Saturday, so he should keep his place in Filipe Coelho's side.
Harrison Murray-Campbell is expected to skipper the visitors, while Ato Ampah, Donnell McNeilly, Travis Akomeah and Richard Olise are others in contention to start.