Newcastle United U21 head to Bradford City on Tuesday in EFL Trophy Northern Section Group H.
Rotherham United top the pool after beating Mansfield Town 2-0 last month.
The game will be shown on Sky Sports+.
Bradford City v Newcastle United U21 kicks off at 19:30 on Tuesday 3rd September at the University of Bradford Stadium.
Bradford City sit ninth in League Two after losing 2-1 at Grimsby on Saturday.
They have a decent recent pedigree in this competition, reaching last season's semi-finals before going down 1-0 to Wycombe.
Newcastle United U21 are 12th in the Premier League 2 table, their most recent outing being Friday's 2-0 defeat at Derby.
Tuesday's tie will be the first time the two have met, while City last faced Newcastle's first team in March 2001, playing out a 2-2 draw.
Summer signing Aden Baldwin faces a spell on the sidelines with a hamstring injury he suffered at Grimsby and Jack Shepherd could take his place.
Olly Sanderson scored off the bench at the weekend and may have persuaded boss Graham Alexander to give him a start.
Newcastle United U21 may offer a glimpse into the future, with boss Neil Winskill expected to recall 17-year-old Adam Harrison in goal.